Publications

2019-

Kurosawa A, Saito S, Sakurai M, Shinozuka M, Someya Y, Adachi N.
Arsenic affects homologous recombination and single-strand annealing but not end-joining pathways during DNA double-strand break repair.
FEBS J. 2023 (in press)

Someya Y, Kobayashi S, Toriumi K, Takeda S, Adachi N, Kurosawa A.
A cell system-assisted strategy for evaluating the natural antioxidant-induced double-stranded DNA break (DSB) style.
Genes (Basel). 2023 Feb 6;14(2):420.

Suzuki T, Sassa A, Gruz P, Gupta RC, Johnson F, Adachi N, Nohmi T.
Error-prone bypass patch by a low-fidelity variant of DNA polymerase zeta in human cells.
DNA Repair (Amst). 2021 Apr;100:103052.

Kurosawa A, Kuboshima H, Adachi N.
Complex genetic interactions between DNA polymerase ѓА and the NHEJ ligase.
FEBS J. 2020 Jan;287(2):377-385.

Duncan JR, Lieber MR, Adachi N, Wahl R.
Reply: Radiation dose does matter: Mechanistic insights into DNA damage and repair support the linear no-threshold model of low-dose radiation health risks.
J Nucl Med. 2019 Mar;60(3):437-438.

2016-2018

Morotomi-Yano K, Saito S, Adachi N, Yano KI.
Dynamic behavior of DNA topoisomerase IIѓА in response to DNA double-strand breaks.
Sci Rep. 2018 Jul 9;8(1):10344.

Duncan JR, Lieber MR, Adachi N, Wahl R.
Radiation dose does matter: Mechanistic insights into DNA damage and repair support the linear no-threshold model of low-dose radiation health risks.
J Nucl Med. 2018 Jul;59(7):1014-1016.

Duncan JR, Lieber MR, Adachi N, Wahl R.
DNA repair after exposure to ionizing radiation is not error-free.
J Nucl Med. 2018 Feb;59(2):348. doi: 10.2967/jnumed.117.197673.

Saito S, Maeda R, Adachi N.
Dual loss of human POLQ and LIG4 abolishes random integration.
Nature Commun. 2017 Jul 11;8:16112. doi: 10.1038/ncomms16112.

Saito S, Kurosawa A, Adachi N.
Mechanistic basis for increased human gene targeting by promoterless vectors: roles of homology arms and Rad54 paralogs.
FEBS J. 2017 Sep;284(17):2748-2763. doi: 10.1111/febs.14137. (selected as the Editor's Choice)

Chang HHY, Pannunzio NR, Adachi N, Lieber MR.
Non-homologous DNA end joining and alternative pathways to double-strand break repair.
Nature Rev Mol Cell Biol. 2017 Aug;18(8):495-506. doi: 10.1038/nrm.2017.48. (selected as the Cover for the August 2017 issue)

Maeda R, Tamashiro H, Takano K, Takahashi H, Suzuki H, Saito S, Kojima W, Adachi N, Ura K, Endo T, Tamura TA.
TBP-like protein (TLP) disrupts the p53-MDM2 interaction and induces long-lasting p53 activation.
J Biol Chem. 2017 Feb 24;292(8):3201-3212.

Kanemaru Y, Suzuki T, Sassa A, Matsumoto K, Adachi N, Honma M, Numazawa S, Nohmi T.
DNA polymerase kappa protects human cells against MMC-induced genotoxicity through error-free translesion DNA synthesis.
Genes Environ. 2017 Jan 7;39:6.

Suzuki T, Gruz P, Honma M, Adachi N, Nohmi T.
The role of DNA polymerase zeta in translesion synthesis across bulky DNA adducts and cross-links in human cells.
Mutat Res. 2016 Sep - Oct;791-792:35-41.

Suzuki T, Gruz P, Honma M, Adachi N, Nohmi T.
Sensitivity of human cells expressing low-fidelity or weak-catalytic-activity variants of DNA polymerase ѓД to genotoxic stresses.
DNA Repair (Amst). 2016 Sep;45:34-43.

Saito S, Kurosawa A, Adachi N.
Mutations in XRCC4 cause primordial dwarfism without causing immunodeficiency.
J Hum Genet. 2016 Aug;61(8):679-85.

Saito S, Adachi N.
Advances in the development of gene-targeting vectors to increase the efficiency of genetic modification.
Biol Pharm Bull. 2016;39(1):25-32.

2013-2015

Saito S, Ura K, Kodama M, Adachi N.
Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection.
BMC Res Notes. 2015 Jun 30;8:278.

Kanemaru Y, Suzuki T, Niimi N, Gruz P, Matsumoto K, Adachi N, Honma M, Nohmi T.
Catalytic and non-catalytic roles of DNA polymerase ѓИ in protection of human cells against genotoxic stresses.
Environ Mol Mutagen. 2015 Oct;56(8):650-62.

Moscariello M, Wieloch R, Kurosawa A, Li F, Adachi N, Mladenov E, Iliakis G.
Role for Artemis nuclease in the repair of radiation-induced DNA double strand breaks by alternative end joining.
DNA Repair (Amst). 2015 Jul;31:29-40.

Ishii T, Hayakawa H, Sekiguchi T, Adachi N, Sekiguchi M.
Role of Auf1 in elimination of oxidatively damaged messenger RNA in human cells.
Free Radic Biol Med. 2015 Feb;79:109-16.

Ishii A, Kurosawa A, Saito S, Adachi N.
Analysis of the role of homology arms in gene-targeting vectors in human cells.
PLoS One. 2014 Sep 24;9(9):e108236.

Sassa A, Suzuki T, Kanemaru Y, Niimi N, Fujimoto H, Katafuchi A, Gruz P, Yasui M, Gupta RC, Johnson F, Ohta T, Honma M, Adachi N, Nohmi T.
In vivo evidence that phenylalanine 171 acts as a molecular brake for translesion DNA synthesis across benzo[a]pyrene DNA adducts by human DNA polymerase kappa.
DNA Repair (Amst). 2014 Mar;15:21-8.

Adachi N, Saito S, Kurosawa A.
Repair of accidental DNA double-strand breaks in the human genome and its relevance to vector DNA integration.
Gene Technology. 2013;3:e107.

Liu S, Liu X, Kamdar RP, Wanotayan R, Sharma MK, Adachi N, Matsumoto Y.
C-terminal region of DNA ligase IV drives XRCC4/DNA ligase IV complex to chromatin.
Biochem Biophys Res Commun. 2013 Sep 20;439(2):173-8.

Kamekawa H, Kurosawa A, Umehara M, Toyoda E, Adachi N.
Endogenous factors causative of spontaneous DNA damage that leads to random integration in human cells.
Gene Technology. 2013;2:105.

Adachi N.
Generation and use of genetically modified human cell lines: a promising approach for in vitro toxicology studies.
J Clinic Toxicol. 2013;3(5):45.

Kurosawa A, Saito S, So S, Hashimoto M, Iwabuchi K, Watabe H, Adachi N.
DNA ligase IV and Artemis act cooperatively to suppress homologous recombination in human cells: Implications for DNA double-strand break repair.
PLoS One. 2013 Aug 14;8(8):e72253.

Suzuki T, Ukai A, Honma M, Adachi N, Nohmi T.
Restoration of mismatch repair functions in human cell line Nalm-6, which has high efficiency for gene targeting.
PLoS One. 2013 Apr 15;8(4):e61189.

Fujita M, Sasanuma H, Yamamoto KN, Harada H, Kurosawa A, Adachi N, Omura M, Hiraoka M, Takeda S, Hirota K.
Interference in DNA replication can cause mitotic chromosomal breakage unassociated with double-strand breaks.
PLoS One. 2013;8(4):e60043.

Cui X, Lu Z, Kurosawa A, Klemm L, Bagshaw A, Tsai A, Gemmell N, Muschen M, Adachi N, Hsieh CL, Lieber MR.
Both CpG methylation and AID are required for the fragility of the human Bcl-2 major breakpoint region: Implications for the timing of the breaks in the t(14;18).
Mol Cell Biol. 2013 Mar;33(5):947-57.

2010-2012

Cowell IG, Sondka Z, Smith K, Lee KC, Manville CM, Sidorczuk-Lesthuruge M, Rance HA, Padget K, Jackson GH, Adachi N, Austin CA.
Model for MLL translocations in therapy-related leukemia involving topoisomerase IIbeta mediated DNA strand breaks and gene proximity.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8989-94.

Malu S, De Ioannes P, Kozlov M, Greene M, Francis D, Hanna M, Pena J, Escalante CR, Kurosawa A, Erdjument-Bromage H, Tempst P, Adachi N, Vezzoni P, Villa A, Aggarwal AK, Cortes P.
Artemis C-terminal region facilitates efficient V(D)J recombination through its interaction with both DNA Ligase IV and DNA-PKcs.
J Exp Med. 2012 May 7;209(5):955-63.

Kohzaki M, Chiourea M, Versini G, Adachi N, Takeda S, Gagos S, Halazonetis TD.
The helicase domain and C-terminus of human RecQL4 facilitate replication elongation on DNA templates damaged by ionizing radiation.
Carcinogenesis. 2012 Jun;33(6):1203-10.

Schrijvers R, De Rijck J, Demeulemeester J, Adachi N, Vets S, Ronen K, Christ F, Bushman FD, Debyser Z, Gijsbers R.
LEDGF/p75-independent HIV-1 replication demonstrates a role for HRP-2 and remains sensitive to inhibition by LEDGINs.
PLoS Pathog. 2012;8(3):e1002558.

Sato R, Iiizumi S, Kim ES, Honda F, Lee SK, Adachi N, Koyama H, Mizutani S, Morio T.
Impaired cell adhesion, apoptosis, and signaling in WASP gene-disrupted Nalm-6 pre-B cells and recovery of cell adhesion using a transducible form of WASp.
Int J Hematol. 2012 Mar;95(3):299-310.

Kurosawa A, Saito S, Mori M, Adachi N.
Nucleofection-based gene targeting in human pre-B cells.
Gene. 2012 Jan 15;492(1):305-8.

Adachi N.
High-efficiency gene targeting in a human pre-B cell line: towards the human gene knockout project.
Genome Biol. 2010 Oct 11;11:P1.

Saito K, Adachi N, Koyama H, Matsushita M.
OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling.
FEBS Lett. 2010 Aug 4;584(15):3340-7.

Kurosawa A, Adachi N.
Functions and regulation of Artemis: A goddess in the maintenance of genome integrity.
J Radiat Res. 2010;51(5):503-9.

Uemura M, Niwa Y, Kakazu N, Adachi N, Kinoshita K.
Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors.
PLoS ONE. 2010 Mar 24;5(3):e9846.

Asagoshi K, Tano K, Chastain PD II, Adachi N, Sonoda E, Kikuchi K, Koyama H, Nagata K, Kaufman DG, Takeda S, Wilson SH, Watanabe M, Swenberg JA, Nakamura J.
FEN1 functions in long patch base excision repair under conditions of oxidative stress in vertebrate cells.
Mol Cancer Res. 2010 Feb;8(2):204-15.

2007-2009

Killen MW, Stults DM, Adachi N, Hanakahi L, Pierce AJ.
Loss of Bloom syndrome protein destabilizes human gene cluster architecture.
Hum Mol Genet. 2009 Sep 15;18(18):3417-28.

Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.
Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.
PLoS Genetics. 2009 Apr;5(4):e1000461.

Toyoda E, Kurosawa A, Kamekawa H, Adachi N.
Topoisomerase IIalpha inhibition following DNA transfection greatly enhances random integration in a human pre-B lymphocyte cell line.
Biochem Biophys Res Commun. 2009 May;382(3):492-6

Nishijima H, Takami Y, Nakayama T, Adachi N, Shibahara K.
Improved applications of the tetracycline-regulated gene depletion system.
Biosci Trends. 2009 Oct;3(5):161-7.

Yano K, Morotomi-Yano K, Adachi N, Akiyama H.
Molecular mechanism of protein assembly on DNA double-strand breaks in the non-homologous end-joining pathway.
J Radiat Res. 2009 Mar;50(2):97-108.

Toyoda E, Kurosawa A, Fujii M, Adachi N.
Heterozygous disruption of the DNA topoisomerase I gene confers cellular resistance to camptothecin in human cells.
Biol Pharm Bull. 2009 Apr;32(4):724-7.

Fujii M, Adachi N, Shikatani K, Ayusawa D.
An [FeFe]-hydrogenase-like gene is involved in the regulation of sensitivity to oxygen in yeast and nematode.
Genes Cells. 2009 Apr;14(4):457-68.

Adachi N, Yano K, Kurosawa A.
Mechanisms of chromosomal DNA strand break repair in human cells.
Tanpakushitsu Kakusan Koso. 2009 Mar;54(4):472-8.

Ono T, Nishijima H, Adachi N, Iiizumi S, Koyama H, Shibahara K.
Generation of tetracycline-inducible conditional gene knockout cells in a human Nalm-6 cell line.
J Biotechnol. 2009 Apr 20;141(1-2):1-7.

Rao VA, Klein SR, Agama KK, Toyoda E, Adachi N, Pommier YG, Shacter EB.
The iron chelator Dp44mT causes DNA damage and selective inhibition of topoisomerase-IIalpha in breast cancer cells.
Cancer Res. 2009 Feb 1;69(3):948-57.

Adachi N, Nishijima H, Shibahara K.
Gene targeting using the human Nalm-6 pre-B cell line.
Biosci Trends. 2008;2(5):169-80.

Iiizumi S, Kurosawa A, So S, Ishii Y, Chikaraishi Y, Ishii A, Koyama H, Adachi N.
Impact of non-homologous end-joining deficiency on random and targeted DNA integration: Implications for gene targeting.
Nucleic Acids Res. 2008 Nov;36(19):6333-42.

Jayaram S, Ketner G, Adachi N, Hanakahi LA.
Loss of DNA Ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
Nucleic Acids Res. 2008 Oct;36(18):5773-86.

Iiizumi S, Adachi N.
Gene targeting in cultured human cells.
Seikagaku. 2008 Jul;80(7):651-7.

Abe T, Ishiai M, Hosono Y, Yoshimura A, Tada S, Adachi N, Koyama H, Takata M, Takeda S, Enomoto T, Seki M.
KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.
Cell Signal. 2008 Nov;20(11):1978-85.

Toyoda E, Kagaya S, Cowell IG, Kurosawa A, Kamoshita K, Nishikawa K, Iiizumi S, Koyama H, Austin CA, Adachi N.
NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform.
J Biol Chem. 2008 Aug 29;283(35):23711-20.

Iwabuchi K, Hashimoto M, Matsui T, Kurosawa A, Adachi N, Date T.
Cell sorting analysis of cell cycle-dependent X-ray sensitivity in end joining-deficient human cells.
Biochem Biophys Res Commun. 2008 Aug 8;372(4):662-7.

Adachi N, Kurosawa A, Koyama H.
Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6.
Methods Mol Biol. 2008;435:17-29.

Kurosawa A, Koyama H, Takayama S, Miki K, Ayusawa D, Fujii M, Iiizumi S, Adachi N.
The requirement of Artemis in double-strand break repair depends on the type of DNA damage.
DNA Cell Biol. 2008 Jan;27(1): 55-61.

Takayama S, Fujii M, Kurosawa A, Adachi N, Ayusawa D.
Overexpression of the HAM1 gene detoxifies 5-bromodeoxyuridene in the yeast Saccharomyces cerevisiae
Curr Genet. 2007 Nov;52(5-6):203-11.

Nomura Y, Adachi N, Koyama H.
Human Mus81 and FANCB independently contribute to repair of DNA damage during replication.
Genes Cells. 2007 Oct;12(10):1111-22.

So S, Adachi N, Koyama H.
Absence of p53 enhances growth defects and etoposide sensitivity of human cells lacking the Bloom syndrome helicase BLM.
DNA Cell Biol. 2007 Jul;26(7):515-25.

2004-2006

Iiizumi S, Nomura Y, So S, Uegaki K, Aoki K, Shibahara K, Adachi N, Koyama H.
Simple, one-week method to construct gene-targeting vectors: application to production of human knockout cell lines.
BioTechniques. 2006 Sep;41(1):311-6.

Iwabuchi K, Hashimoto M, Matsui T, Kurihara T, Shimizu H, Adachi N, Ishiai M, Yamamoto H, Tauchi H, Takata M, Koyama H, Date T.
53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis.
Genes Cells. 2006 Aug;11(8):935-48.

So S, Nomura Y, Adachi N, Kobayashi Y, Hori T, Kurihara Y, Koyama H.
Enhanced gene targeting efficiency by siRNA that silences the expression of the Bloom syndrome gene in human cells.
Genes Cells. 2006 Apr;11(4):363-71.

Hochegger H, Dejsuphong D, Fukushima T, Morrison C, Sonoda E, Schreiber V, Zhao GY, Saberi A, Masutani M, Adachi N, Koyama H, de Murcia G, Takeda S.
Parp-1 protects homologous recombination from interference by Ku and Ligase IV in vertebrate cells.
EMBO J. 2006 Mar 22;25(6):1305-14.

Adachi N, So S, Iiizumi S, Nomura Y, Murai K, Yamakawa C, Miyagawa K, Koyama H.
The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination.
DNA Cell Biol. 2006 Jan;25(1):19-24.

Uegaki K, Adachi N, So S, Iiizumi S, Koyama H.
Heterozygous inactivation of human Ku70/Ku86 heterodimer does not affect cell growth, double-strand break repair, or genome integrity.
DNA Repair (Amst). 2006 Mar 7;5(3):303-11.

Kikuchi K, Taniguchi Y, Hatanaka A, Sonoda E, Hochegger H, Adachi N, Matsuzaki Y, Koyama H, van Gent DC, Jasin M, Takeda S.
Fen-1 facilitates homologous recombination by removing divergent sequences at DNA break ends.
Mol Cell Biol. 2005 Aug;25(16):6948-55.

Adachi N, Iiizumi S, Koyama H.
Evidence for a role of vertebrate Rad52 in the repair of topoisomerase II-mediated DNA damage.
DNA Cell Biol. 2005 Jun;24(6):388-93.

Irvine RA, Adachi N, Shibata DK, Cassell GD, Yu K, Karanjawala ZE, Hsieh CL, Lieber MR.
Generation and characterization of endonuclease G null mice.
Mol Cell Biol. 2005 Jan;25(1):294-302.

So S, Adachi N, Lieber MR, Koyama H.
Genetic interactions between BLM and DNA ligase IV in human cells.
J Biol Chem. 2004 Dec 31;279(53):55433-42. Epub 2004 Oct 26.

Adachi N, So S, Koyama H.
Loss of nonhomologous end joining confers camptothecin resistance in DT40 cells. Implications for the repair of topoisomerase I-mediated DNA damage.
J Biol Chem. 2004 Sep 3;279(36):37343-8.

Adachi N, Iiizumi S, So S, Koyama H.
Genetic evidence for involvement of two distinct nonhomologous end-joining pathways in repair of topoisomerase II-mediated DNA damage.
Biochem Biophys Res Commun. 2004 Jun 11;318(4):856-61.

2001-2003

Adachi N, Suzuki H, Iiizumi S, Koyama H.
Hypersensitivity of nonhomologous DNA end-joining mutants to VP-16 and ICRF-193: implications for the repair of topoisomerase II-mediated DNA damage.
J Biol Chem. 2003 Sep 19;278(38):35897-902.

Akimitsu N, Adachi N, Hirai H, Hossain MS, Hamamoto H, Kobayashi M, Aratani Y, Koyama H, Sekimizu K.
Enforced cytokinesis without complete nuclear division in embryonic cells depleting the activity of DNA topoisomerase IIalpha.
Genes Cells. 2003 Apr;8(4):393-402.

Karanjawala ZE, Adachi N, Irvine RA, Oh EK, Shibata D, Schwarz K, Hsieh CL, Lieber MR.
The embryonic lethality in DNA ligase IV-deficient mice is rescued by deletion of Ku: implications for unifying the heterogeneous phenotypes of NHEJ mutants.
DNA Repair (Amst). 2002 Dec 5;1(12):1017-26.

Adachi N, Karanjawala ZE, Matsuzaki Y, Koyama H, Lieber MR.
Two overlapping divergent transcription units in the human genome: the FEN1/C11orf10 locus.
OMICS. 2002;6(3):273-9.

Matsuzaki Y, Adachi N, Koyama H.
Vertebrate cells lacking FEN-1 endonuclease are viable but hypersensitive to methylating agents and H2O2.
Nucleic Acids Res. 2002 Jul 15;30(14):3273-7.

Adachi N, Lieber MR.
Bidirectional gene organization: a common architectural feature of the human genome.
Cell. 2002 Jun 28;109(7):807-9.

Fujii M, Ito H, Hasegawa T, Suzuki T, Adachi N, Ayusawa D.
5-Bromo-2'-deoxyuridine efficiently suppresses division potential of the yeast Saccharomyces cerevisiae.
Biosci Biotechnol Biochem. 2002 Apr;66(4):906-9.

Adachi N, Ishino T, Ishii Y, Takeda S, Koyama H.
DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: Implications for DNA double-strand break repair.
Proc Natl Acad Sci U.S.A. 2001 Oct 9;98(21):12109-13.

Kobayashi M, Adachi N, Aratani Y, Kikuchi A, Koyama H.
Decreased topoisomerase IIalpha expression confers increased resistance to ICRF-193 as well as VP-16 in mouse embryonic stem cells.
Cancer Lett. 2001 May 10;166(1):71-7.

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